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HPLC, LC-MS and UHPLC
Yes, HPLC and LC-MS grade solvents are filtered through 0,22 µm. UHPLC-MS grade solvents are filtered through 0,1 µm.
Yes, LC-MS grade blends of acetonitrile, methanol and water are available.
Yes, a wide range of LC-MS grade additives are available should customers want to prepare the blends themselves.
The Reserpine Test and Digoxine Test is performed in order to verify that a solvent is pure enough to be used in HPLC techniques such as LC / MS and UHPLC / MS. With the Reserpine Test the solvent is considered to be pure and validated for use in the above techniques when no impurities are detected on either side of the Reserpine [JK Note: should this be Reserpine peak?].
We recommend that where pre-filtered HPLC/UHPLC/LC-MS/ UHPLC-MS solvents are used, they not be filtered prior to loading unless the user has blended them with modifiers. Since filtration can be a source of contamination , apart fom when blending has occurred, filtering pre filtered solvents is totally unnecessary.
Gas Chromatography
Since the mobile phase is a gas, for general GC analysis, a reagent grade is usually appropriate for the sample preparation. For specific application analysis or for those working with an MS detector, Scharlau has specific grades, such as GC residue analysis and Ultratrace grades. For those analyzing residual solvents by GC-Headspace, Scharlau has also a specific GC-Headspace grade.
Since Reagent Grade solvents are usually controlled according to ACS, which does require residual solvent analysis to be controlled, you can never be sure if the solvent is free from residues. Thus the reliability of your results is questionable were you to use Reagent Grade solvents.
No. Methanol is a Class 2 solvent according to ICH Guidelines, so it is important to make sure the sample is free from methanol. Using it as a solvent will lead to incorrect results.
These grades differ in the way they’re certified for the absence of impurities. The specifications to meet are conditioned by the type of residues present in the samples and the technology used for those residues detection.
The GC solvents for Residue Analysis, are used in the in the preparation of vegetal specimens, in the analysis of pesticides where solvent extraction is required.
The residues extracted use to be organochlorinated, dioxin, furan and PCB pesticides, and they’re commonly detected after GC with an EDC detector.
Thus, this grade of solvents undergo a GC-EDC analysis for assessing absence of impurities within the common working range (see the max. impurity levels allowed here GC Residue Analysis)
In the other hand, the GC solvents for Ultra-Trace analysis are used for organic residue extraction/concentration procedures in environmental samples.
The samples are analized for high, medium and low volatile halogenated hydrocarbons and polyaromatic hydrocarbons, that are commonly detected after GC by FID detector additionally to EDC detection.
Our GC Ultra-Trace grade solvents have the tightest specification, as they’re tested by GC-EDC and GC-FID to assess the absence of impurities within the common working range (see the max. impurity levels allowed here GC Ultra-Trace Analysis)
Aquagent® Karl Fischer Titration
Yes. Aldehydes and ketones when in contact with methanol produce water as a side reaction. Thus you need to use a specific reagent free from methanol.
Yes, the KF reaction takes place at pH 5-7, running optimally at pH(?5) [JK Note: is pH 5 the optimium?) A buffer is required to increase the pH to 5 or higher for the reaction to take place properly.
The reason for using Methanol Fast in conjunction with the Complet reagent[JK note: ? is this the correct spelling] is to speed-up the titration reaction. While we have not confiirmed the success or otherwise of using Methanol Fast in conjunction with pyridine, we do not recommend this technique. [JK note” I am not sure if I have captured the intent of this sentence?]
The reagents are formulated to work best when combined. However we never recommend that products from different brands be mixed.
There are 3 different Aquagent® standards: – Aquagent® Standard 1.0 AQ0019: contains 1,000 ppm of water; it is the formualtion that contains the least amount of water and is used to check the reliability of KF Coulometirc titrators. Aquagent® Standard 1,0 is traceable to NIST. – Aquagent® Standard 5.0 AQ0021: contains 5,000 ppm of water and is used to check the reliability of KF Volumetric titrators. Because this standard is used for the routine calibration of equipment, it does not require a high level of accuracy and therefore, it is not traceable to NIST. – Aquagent® Standard 10.0 AQ0020: contains 10,000 ppm of water and is used to re-titrate both the Complet 5 (1ml of reagent reacts with 5 mg of water) and Complet 2 (1ml of reagent reacts with 2 mg of water) reagent. Because this standard contains 10,000ppm of water, there is sufficient water available in the formulation to re-titrate both the Complet 5 and Complet 2 reagents.
IThis product is used together with cells with diaphragm, since it is an anolyte. It works when paired with Aquagent® Coulometric CG AQ0023, which is the catholyte AQ00 Aquagent® Coulometric Oil is incorporated into the anode compartment, which is separated from the cathode compartment by the membrane.
The two-component system is superior to the one-component system because the end-point is reached faster, it has a more stable end point and it does not require as frequent re-titration (due to the reagents being separated).
No, a Karl Fischer reagent must always be combined with a solvent in order to work.
For each liter of KF solvent you will need one liter of KF reagent. The relationship is 1:1. The Solvent is neutralized before each measure, allowing to be reused several times.
Ultratrace® Ultrapure Acids
While to some extent it depends on the sample, since GFAAS has a very low detection limit, our recommendation is to use a ppt grade.
Yes, a ppb grade should be enough. Our ppb grade acids have a maximum of 1 ppb of metal traces, which should be sufficient, given that the instrument’s detection limit is usually in the region of 1 – 10 ppb.
Other
Previously we used a blue indicator that contained cobalt chloride however this is highly toxic and carcinogenic. As such, this chemical was banned in Europe therefore it should no longer be used due to the health and saftey concerns.
All Scharlau products are for laboratory use with the exception of the Pharmpur grades, which are suitable for use as raw materials in drug manufacture. None of the products however, have Food Grade certificates.
pH buffer solutions are offered for use at two different temperatures – namely 20ºC and 25ºC, this being due to variances in the requirements of different geographical markets. In general, the European market uses 20ºC while in America they use 25ºC. European legislation generally requires that measurements be performed at 20ºC, whereas US regulations requires them at 25ºC . Therefore in order to comply with both the European and US legislation and to adapt to the two geographical requirements, we offer buffer solutions that are suitable for use at both temperatures.
The shelf-life ot any product refers to the period until the expiry date or to the date from when the product package is opened. Once the pagkage is open, it is imposible for the manufacturer to take any further responsibility for the condition of the product. due to their having no control over the transportation, storage and handling conditions.
The expiration of any product always refers to the period from when the product has been manufactured and released, until the package is opened, or when the expiry date as shown on the label (or in the CoA) is achieved. Once the package is opened, the shelf life is no longer guaranteed by the manufacturer even when the expiry date has not been reached. No manufacturer can ensure a shelf life after the package is opened.
Scharlab includes the quality release date in all the Certificates of Analysis, along with the expiry date.
When did Scharlau commence incorporating the safety valve in the caps for hydrogen peroxide bottles?
Since June 20
This depends on the pack size, however full boxes will contain 4 or 6 units. As a general rule, pack sizes of 1L, 1Kg or smaller are supplied as 6 units per box, while 2,5L and 4L bottles being of a larger size are supplied as 4 units per box.
Scharlau bottles ranging from 500ml up to 7L have a standard bottleneck GL45, which is suitable for use with bottle top dispensers. For solids up to 1 Kg, the bottleneck size is GL54.
HDPE containers can transfer phthalates and other plasticizers to the solvent, due to these being large molecules that can damage columns and pumps and so interfere with the analytical results. This is the reason that HPLC grade solvents from Scharlau are only available in glass bottles or in selected countries, supplied in drums.
Despite being sealed, anhydrous solvents are susceptible to moisture. This is the reason that Scharlau’s anhydrous solvents are supplied in vacuum-sealed foil bags, which also provides additional protection to ensure that the products are maintained in optimum condition prior to use.
The UN number is a number assigned by the United Nations to goods which are considered dangerous for transport.
Chemicals that are considered to be dangerous goods are regulated in Europe by three guidelines IATA for air transport, ADR for land transport and IMDG for shipping.
While both are information storage systems, datamatrix is the more modern system and enables the storage of larger amounts of information.
The expiry date is not a requirement under the guidelines and regulations for chemcials Therefore, the manufacturers are not obliged to display it. Sharlau was one of the first companies to include expiry dating on their labels.
The CAS Registry Number is a unique numerical identifier assigned by the Chemical Abstracts Service (part of ACS) to every chemical described in scientific literature and includes amoung others, elements, isotopes, organic and inorganic compounds, ions, and metals among others. A CAS Registry Number is separated by hyphens into three parts, the first consisting of up to 7 digits, the second consisting of two digits, and the third consisting of a single digit serving as a check digit.
What is the smaller one for? The smaller label is both for security and for internal use. The right of the label contains a hologram that guarantees the product authenticity, while the data matrix and numbers adjacent to the hologram are used for production and traceability, however they do not contain anyinformation which is relevant to the end user. relevant information for the end user. All theinformation that the customer should require is included in the main label
Yes, all Scharlau products have a Certificate of Analysis for each batch, that are available online at www.scharlab.com. Some standard reference materials such as pH buffers have the Certificate of Analysis enclosed with the bottles.